Horny Layer Lipids

Formation of Horny Cells

Epithelial cells changing from the granular stage of differentiation to the horny stage are more numerous, and reveal sequential events of transformation in finer detail in the rumen epithelium than in other keratinizing epithelia thus far studied in the electron microscope. Studies of such cells indicate that transformation is initiated by the release of hydrolytic enzymes, as evidenced by the...

Thin-layer Chromatography of Lipids

There has been a tendency to discount thin-layer chromatography (TLC) in recent years because of the availability of high-performance liquid chromatography (HPLC) methods that appeared to offer greater versatility and ease of quantification, amongst other reasons. However, TLC has made a comeback, largely because of the high reproducibility and lower costs of commercial pre-coated plates. It is...

A Case of Horny Papilloma

Horny Growth Arising from Neglected Sebaceous Cyst

One Imam Wd. Noorahamad, aged about 45 years, a private servant, was admitted into the Civil Hospital, Belgaum, on the 17th February 1902. Previous History?The patient stated thaf. since birth he had a small painless tumour of the size of a gram at the root of the neck on the right side. This gradually grew larger and larger till it was finally the size of a small cocoanut. About two years ago ...

Thin-layer chromatography of tissue lipids without extraction.

Frozen tissue sections were applied directly to silica gel plates, and the lipids were separated by developing the plates in different solvent systems. Quantitation of the lipid classes was achieved by direct transmission densitometry after the plates were treated with a chromic acid-sulfuric acid spray.